Characterization of two flavonol synthases with iron-independent flavanone 3-hydroxylase activity from Ornithogalum caudatum Jacq
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چکیده
منابع مشابه
Genetic Control of Flavanone 3-Hydroxylase Activity and Flavonoid 3'-Hydroxylase Activity in Antirrhinum majus (Snapdragon)
Anthocyanin Biosynthesis, Antirrhinum majus, Flavonoids, Flavanone 3-Hydroxylase, Flavonoid 3'-Hydroxylase, Genetic Control In flower extracts of defined genotypes of Antirrhinum majus, two different hydroxylases were found catalysing the hydroxylation of naringenin and eriodictyol in the 3-position and of naringenin in the 3'-position. The 3-hydroxylase is a soluble enzyme and belongs accordin...
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A key step in the biosynthesis of flavonoids is the hydroxylation of (2s)-flavanones in the 3 position to form the respective dihydroflavonols. The reaction is catalyzed by F30H, a soluble fraction enzyme requiring 2-oxoglutarate, oxygen, Fez+, and ascorbate for full activity (Heller and Forkmann, 1988). By using genetic mutants that lack F 3 0 H activity, cDNA clones have been identified that ...
متن کاملCloning and characterization of a flavanone 3-hydroxylase gene from Ginkgo biloba.
Flavanone 3-hydroxylase (F3H) activity is necessary for the biosynthesis of flavonoids, the main ingredients of Gingko biloba extract. The full-length cDNA and genomic DNA sequences of F3H gene were isolated from G. biloba for the first time. The full-length cDNA of G. biloba F3H gene (designated as GbF3H) contained a 1071 bp open reading frame (ORF) encoding a 357-amino-acid protein with a cal...
متن کاملcDNA Isolation and Functional Characterization of UDP-d-glucuronic Acid 4-Epimerase Family from Ornithogalum caudatum.
d-Galacturonic acid (GalA) is an important component of GalA-containing polysaccharides in Ornithogalum caudatum. The incorporation of GalA into these polysaccharides from UDP-d-galacturonic acid (UDP-GalA) was reasonably known. However, the cDNAs involved in the biosynthesis of UDP-GalA were still unknown. In the present investigation, one candidate UDP-d-glucuronic acid 4-epimerase (UGlcAE) f...
متن کاملFunctional Analysis of Two Flavanone-3-Hydroxylase Genes from Camellia sinensis: A Critical Role in Flavonoid Accumulation
Flavonoids are major secondary metabolites in Camellia sinensis. Flavanone-3-hydroxylase (F3H) is a key enzyme in flavonoid biosynthesis in plants. However, its role in the flavonoid metabolism in C. sinensis has not been well studied. In this study, we cloned two F3Hs from C. sinensis, named CsF3Ha and CsF3Hb, where CsF3Ha containing 1107 bases encoded 368 amino acids, and CsF3Hb containing 10...
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ژورنال
عنوان ژورنال: BMC Plant Biology
سال: 2019
ISSN: 1471-2229
DOI: 10.1186/s12870-019-1787-x